AChE overexpression mediated by oncolytic adenovirus ZD55 inhibited cancer cell growth. (A) Western blot detection of AChE expression in AGS cells infected with adenoviruses for 48 hours. GAPDH was used as an internal control. (B) 48 hours after AGS cells were infected with ZD55-AChE, AChE activity in the medium (left) and cell lysates (right) were detected at the indicated time points. Data are presented as mean ± SD (n = 3). (C) Cell viability of AGS cells 4 days after the indicated MOI of ZD55-AChE infection measured by MTT assay. (D) ZD55-AChE suppressed AGS cell growth at a MOI of 2 as measured by MTT assay. (E) Cell viability of NCI-N87 and MGC80-3 cells 4 days after the indicated MOI of ZD55-AChE infection measured by MTT assay. (F) Detection of the cytotoxicity of ZD55-AChE on GES-1 cells 4 days after infection by MTT assay. (G) Tumor growth curve of the adenoviruses-treated subcutaneous MGC80-3 xenografts. Day 0, animals were intratumorally injected with saline or adenoviruses. Data are expressed as mean ± SD (n = 6). MTT experiments were repeated 3 times. Data in C-F are shown as fold change relative to that of mock-treated cells. All data shown represent mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, NS: non-significant.