Replication-deficient adenovirus expressing AChE inhibited gastric cancer cell growth. (A) Western blot analysis of AChE expression in Ad.AChE infected AGS cells. GAPDH was used as an internal control. *, non-specific band. (B) 48 hours after AGS cells were infected with Ad.AChE, AChE activity in the medium (left) and cell lysates (right) were detected at the indicated time points. Data are presented as mean ± SD (n = 3). (C) Cell viability of AGS and NCI-N87 cells 4 days after the indicated MOI of adenovirus infection measured by MTT assay. (D) Ad.AChE suppressed AGS cell growth at a MOI of 100 as measured by MTT assay. (E) Detection of the cytotoxicity of Ad.AChE on GES-1 cells by MTT assay. MTT experiments were repeated 3 times. Data in C-E are shown as fold change relative to that of mock-treated cells. All data shown represent mean ± SD (n = 3). **P < 0.01, NS: non-significant.