The immunosignature distinguishes canine lymphoma patients from healthy dogs. A Student’s T-test (p < 0.05 with FDR) and a 1.5 fold change between classes was used to select 340 informative peptides. The distribution of intensities is shown in the Heatmap (A). Colors represent the per peptide median normalized intensities. Yellow indicates the median, red five-fold above the median and blue 0.25 fold below the median. Each row represents a peptide and each column represents and individual. Individuals were clustered in GeneSpring using the Pearson correlation to each other. Variation among individuals based on the 340 peptides is shown in the PCA plot (B) where the first two principal components are plotted. The classification efficacy is plotted in the ROC curve in (C). Print run one and the KPL conjugate were used for this assay.