Figure 4From: MiR-107 and miR-99a-3p predict chemotherapy response in patients with advanced colorectal cancer 3′-UTR reporter assay for miR target validation. HEK 293 cells were transfected with luciferase reporter vector containing the 3′-UTR region of CCDN1. Reporter vectors were co-transfected with a miR-107 mimic or control miRNA mimic (miR NC). Following 24 h incubation, luciferase activity was measured.Back to article page