Table 1 Information on primers used in RT-PCRs
From: Intratumoral heterogeneity impacts the response to anti-neu antibody therapy
Genes | NCBI No. | Forward (5′-3′) | Reverse (5′-3′) | Size(bp) |
|---|---|---|---|---|
MMP1a | NM_032006 | AGACTTCTCTGGTTGCCG | AGAGCCTCCAATCACTGTGC | 210 |
MMP2 | NM_008610 | CTATTCTGTCAGCACTTTGG | CAGACTTTGGTTCTCCAACTT | 309 |
MMP3 | NM_010809 | TGTACCAGTCTACAAGTCCTCCA | CTGCGAAGATCCACTGAAGAAGTAG | 659 |
MMP7 | NM_010810 | CTGCCACTGTCCCAGGAAG | GGGAGAGTTTTCCAGTCATGG | 175 |
MMP8 | NM_008611 | TGACTCTGGTGATTTCTTGCTAA | GTGAAGGTCAGGGGCGATGC | 164 |
MMP9 | NM_013599 | CTCAGAGATTCTCCGTGTCCTGTA | GACTGCCAGGAAGACCTTGGTTA | 241 |
MMP10 | NM_019471 | AGTTGCTCCTGCATGTTCTG | TGCATCCTCTCACCTACTGC | 120 |
MMP11 | NM_008606 | CCGGAGAGTCACCGTCATC | GCAGGACTAG GGACCCAATG | 110 |
MMP14 | NM_008608.3 | CTGATGACGATCGCCGTGGCATCC | GCGTCTGAAGAAGAAGACAGCGAGG | 878 |
CDH1 | NM_009864 | CCATTTTCACGCGCGCTG | CGCGAGCTTGAGATGGAT | 396 |
CDH2 | NM_007664 | AGCGCAGTCTTACCGAAGG | TCGCTGCTTTCATACTGAACTTT | 110 |
Krt18 | NM_010664 | CAGCCAGCGTCTATGCAGG | CCTTCTCGGTCTGGATTCCAC | 123 |
Claudin1 | NM_016674 | GGGGACAACATCGTGACCG | AGGAGTCGAAGACTTTGCACT | 100 |
Occludin | NM_008756 | TTGAAAGTCCACCTCCTTACAGA | CCGGATAAAAAGAGTACGCTGG | 129 |
Ctnna1 | NM_009818 | AAGTCTGGAGATTAGGACTCTGG | ACGGCCTCTCTTTTTATTAGACG | 115 |
Ctnnb1 | NM_007614 | ATGGAGCCGGACAGAAAAGC | CTTGCCACTCAGGGAAGGA | 108 |
Jup | NM_010593 | TGGCAACAGACATACACCTACG | GGTGGTAGTCTTCTTGAGTGTG | 135 |
DDR2 | NM_022563 | ATCACAGCCTCAAGTCAGTGG | TTCAGGTCATCGGGTTGCAC | 116 |
Fibronectin | NM_010233 | AGAGCAAGCCTGAGCCTGAAG | TCGCCAATCTTGTAGGACTGACC | 192 |
FOXC2 | NM_013519 | AACCCAACAGCAAACTTTCCC | GCGTAGCTCGATAGGGCAG | 130 |
S100a4 | NM_011311 | TGAGCAACTTGGACAGCAACA | TTCCGGGGCTCCTTATCTGGG | 124 |
SNAI1 | NM_011427 | CACACGCTGCCTTGTGTCT | GGTCAGCAAAAGCACGGTT | 133 |
SNAI2 | NM_011415 | TGGTCAAGAAACATTTCAACGCC | GGTGAGGATCTCTGGTTTTGGTA | 131 |
Acta2 | NM_007392 | GTCCCAGACATCAGGGAGTAA | TCGGATACTTCAGCGTCAGGA | 102 |
Twist1 | NM_011658 | GGACAAGCTGAGCAAGATTCA | CGGAGAAGGCGTAGCTGAG | 146 |
Vimentin | NM_011701 | CGGCTGCGAGAGAAATTGC | CCACTTTCCGTTCAAGGTCAAG | 124 |
GAPDH | NM_008084 | TTCACCACCATGGAGAAGGC | GGCATGGACTGTGGTCATGA | 250 |