Figure 4
Whole genome microRNA array revealed the differentially regulated microRNAs among virgin and parous ALDH positive MECs. Primary rat MECs were obtained after cell dissociation of the mammary gland tissue from virgin and parous animals. The primary cells were subjected to ALDH staining followed by flow cytometric sorting of ALDHbright cell population. RNA was extracted and cDNA (small microRNA specific) was prepared from these cells. The cDNA was then used to perform whole genome microRNA profiling (n = 6) for ALDH positive MECs from both the groups. MicroRNAs that were 2 folds up-or-down-regulated between the groups were considered as differentially regulated. A) microRNAs in ALDH positive MECsof parous compared to the virgin glands with a fold down regulation of 0.01 and 0.1. B) Sub graph showing the microRNAs which had a fold down regulation between 0.1 and 0.5. This group had the most number of down regulated microRNAs from the dataset. C) Up regulated microRNAs in ALDH positive MECs of the parous compared to the virgin glands which had a fold up regulation > 2 through 15. D) Sub group of microRNAs which had a fold up regulation of >15.