Figure 1
TGF-β1 increased intracellular ROS production and CTGF, NOX-4 and α-SMA mRNA expression. Time-course evaluation of CTGF, NOX-4 and α-SMA mRNA expression performed by qPCR. RMF-EG cells cultures, previously deprived of serum were stimulated with 5 ng/ml of TGF-β1 and their mRNA levels were evaluated at the indicated times. In a parallel group of cells, intracellular ROS production was assessed by measuring fluorescence generated after H2DCFDA incubation. Figure is representative of three independent experiments. Values were normalized by number of viable cells at the end of the experiment.