Figure 6

Invasion and migration assays in HCT116 mock cells and antisense-uPAR HCT116 cells by knockdown of HSP70 and/or MRJ. (A) Transwell assays. Cells were transfected with psiSC, or psiMRJ, or psiHSP70, or psiMRJ plus psiHSP70 and starved for 12 hours then applied to the upper compartment of the transwell chamber. HCT116 mock cells (upper lane) and antisense-uPAR HCT116 cells (lower lane) migrated to the lower surface of the membrane were fixed and stained with 0.1% crystal violet. Representative images were shown. The experiments were repeated for three times. (B) Wound healing assays. HCT116 mock cells (upper lanes) and antisense-uPAR HCT116 cells (lower lanes) were transfected with psiSC, or psiMRJ, or psiHSP70, or psiMRJ plus psiHSP70. Photographs were taken immediately and after 48 hours of creating the scratch. Images shown are representative of three independent experiments.