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Figure 4 | BMC Cancer

Figure 4

From: Heat shock proteins HSP70 and MRJ cooperatively regulate cell adhesion and migration through urokinase receptor

Figure 4

Co-localization of HSP70 with uPAR in cytoplasm. (A) uPAR and HSP70 co-localized in cytoplasm of HEK293-uPAR cells. Cells were fixed and then permeabilized with 0.5% Triton X-100 to show the total uPAR localization (down lane). The cells were then immune-stained with 1% BSA as a negative control (top lane) or with anti-uPAR antibody (in red) and anti-HSP70 antibody (in green) and stained with Alexa Fluor 488 (uPAR) and Alexa Fluor 594 (HSP70) conjugated secondary antibodies at room temperature. Co-localization appeared in yellow color. (B) Co-locolization of exogenous soluble uPAR and endogenous HSP70 in Hela cells. Cells transfected with PRK5 vector (upper lane) or soluble uPAR-His-tag (lower lane) were fixed and then permeabilized with 0.5% Triton. The cells were then immunostained with anti-His-tag antibody (in red) and anti-HSP70 antibody (in green) and stained with Alexa Fluor 488 (uPAR) and Alexa Fluor 594 (HSP70) conjugated secondary antibodies at room temperature, co-localization appeared in yellow color. Data shown are representative of three independent experiments.

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