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Figure 3 | BMC Cancer

Figure 3

From: GPC3 reduces cell proliferation in renal carcinoma cell lines

Figure 3

Effect of GPC3 on colony formation. The GPC3 gene suppresses the colony formation in ACHN (A) and 786-O (B) cell lines. Tumor cell proliferation was assessed in vitro. Cells were transiently transfected with pcDNA3.1 (empty vector) or pcDNA3.1/GPC3 and replated 24 h post-transfection for selection with Geneticin/G418. After 14 days of selection, colonies were stained with Giemsa and counted. Data are presented as the mean of two independent experiments ± SEM. Group comparisons were carried out using Student’s t test, *p < 0.01.

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