Figure 2

Effects of MIF knockdown on cell cycle and apoptosis. DNA content analysis using flow cytometry was performed on (A) MelCV and (B) Me1007 cells after 3d of transfection with control (NC) or MIF siRNA duplexes. Representative profiles show the estimated percentages of cells in gates representing G0/1 (G1), S and G2/M (G2) phases. Only cells with intact DNA contents (2n-4n) were analysed. Apoptotic rates were also estimated as the percentage of total cells present in the sub-G0/G1 region. The percentage of apoptotic cells was estimated from (C) MelCV and (D) Me1007 cells after 3d of transfection with MIF or control siRNA duplexes. The histograms represent the means +/-S.E.M. from 3 replicates (***p < 0.001 and *p < 0.05 comparing between treatments using Student’s t-test). Similar results were obtained in three independent experiments.