Figure 1

Small interfering RNA (siRNA) knockdown of MIF decreases melanoma cell proliferation and viability. Melanoma cell lines were transfected with MIF siRNA duplexes (50 nM MIF-25) or negative control (NC) duplexes under the same conditions. Cellular MIF levels measured in both (A) MelCV and (B) Me1007 cell lines using Western blot show sequential reductions in MIF protein after transfection that were sustained for 4–5 days. Cell number and viability were determined in corresponding samples of MelCV (C and E respectively) and Me1007 (D and F, respectively). Results show both MelCV and Me1007 showed a significant reduction in the cell number starting from day 3 after transfection with viability also reduced in a time-dependent manner. Values are mean +/-SEM (n = 3, ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05 comparing between NC and MIF siRNA transfected cells using Student’s t-test).