Figure 3

2-24a/Cu induces oxidative stress in U2os cells and HeLa cells. (A–C) U2os cells were treated with DMSO, 10 μM copper, 10 μM 2-24a, 2 μM 2-24a/Cu, or an additional 4 mM NAC for 12 h. After incubation with 10 μM H₂DCFDA, cells were washed and examined by fluorescence microscope (A) or FACS (B). The average fluorescent intensity from DCF is indicated (C). (D–F) HeLa cells were treated as indicated. Means ± SD, n = 3. *P < 0.01.