Figure 2

Immunohistochemical detection of NOTCH1 and its ligands DLL1 and JAG1 in a benign bladder tissue. In this representative benign urothelium (corresponding normal tissue from case #15) NOTCH1 and DLL1 are localized in the cytoplasm and plasma membranes of umbrella cells and the membranes predominantly of intermediate cells. Staining in the basal layer is weak, especially for NOTCH1. JAG1 is conversely detectable more strongly in the lower cell layers, mostly in the cytoplasm and weakly at the plasma membrane.