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Figure 5 | BMC Cancer

Figure 5

From: Synergetic cytotoxic activity toward breast cancer cells enhanced by the combination of Antp-TPR hybrid peptide targeting Hsp90 and Hsp70-targeted peptide

Figure 5

Treatment with Antp-TPR in the presence of R11-Hsp70 did not increase glutathione (GSH) concentration but affected ATP dynamics in cancer cells. (A) BT20 cells were treated with or without Antp-TPR (20 μM), R11-Hsp70 (10 μM), or a combination of these peptides for 24 h and subjected to a GSH assay using a luminometer as described in the Materials and Methods section. A total of 0.5 μM 17-AAG was used as a positive control for the increase in GSH concentration in cancer cells. Data are expressed as the means ± SD. (B) BT20 cells were transiently transfected with a pGL4.50 vector and subjected to time-course analysis of ATP dynamics on single-cell imaging using a LV200 system after treatment with or without Antp-TPR, R11-Hsp70, or a combination of these peptides.

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BMC Cancer

ISSN: 1471-2407

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