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Figure 2 | BMC Cancer

Figure 2

From: Synergetic cytotoxic activity toward breast cancer cells enhanced by the combination of Antp-TPR hybrid peptide targeting Hsp90 and Hsp70-targeted peptide

Figure 2

Time course of synergetic cytotoxic activity toward and intracellular penetration of Antp-TPR in cancer cells in combination with R11-Hsp70. (A) Time course of cell viability after treatment with Antp-TPR in the presence or absence of R11-Hsp70. MDA-MB-231 and BT20 cells were treated with Antp-TPR (20 μM) in the presence or absence of R11-Hsp70 (10 μM) for the indicated times and analyzed for cell viability by WST-8 assay. Data are expressed as the means ± SD from triplicate determinations. (B) Intracellular penetration of Antp-TPR and R11-Hsp70. BT20 cells were incubated with Antp-TPR (10 μM) labeled with carboxytetramethyl rhodamine (TAMRA; Antp-TPR-TAMRA) and R11-Hsp70 (10 μM) labeled with fluorescein isothiocyanate (FITC; R11-Hsp70-FITC) for the indicated times. Cells were analyzed by differential interference contrast (DIC) or fluorescence (FITC-green or TAMRA-red), or as merged images (FITC-green and TAMRA-red). All images were obtained by confocal microscopy as described in the Materials and Methods section. All scale bars are 50 μm.

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BMC Cancer

ISSN: 1471-2407

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