Skip to main content
Figure 1 | BMC Cancer

Figure 1

From: Synergetic cytotoxic activity toward breast cancer cells enhanced by the combination of Antp-TPR hybrid peptide targeting Hsp90 and Hsp70-targeted peptide

Figure 1

Increase in the cytotoxic activity toward breast cancer cells of Antp-TPR hybrid peptide in the presence of heat shock protein (Hsp) 70-targeted peptide. (A) Viability of four breast cancer cell lines (MDA-MB-231, BT20, BT474, and MDA-MB-361) treated with Antp-TPR hybrid peptide in the presence or absence of R11-Hsp70 peptide. Cells were incubated with Antp-TPR at the indicated concentrations in the presence or absence of R11-Hsp70 (10 μM) for 24 h and analyzed for cell viability as described in the Materials and Methods section. Data are expressed as the means ± SD from triplicate determinations. (B) Analysis of Hsp90, Hsp70, Akt, and p53 expression in breast cancer and normal cell lines. Cell extracts from the indicated breast cancer and normal cell lines were examined for Hsp90, Hsp70, Akt, and p53 expression by western blotting with corresponding antibodies. β-Actin was used as the loading control. Bands were visualized by chemiluminescence as described in the Materials and Methods section.

Back to article page