Detection of loss of mitochondrial trans-membrane depolarization potential upon BT treatment. Cell lines were treated with 100 μM BT for 24 hrs. Mitochondrial potential was determined by flow cytometry using Rhodamne-123. Data was acquired by BD’s Accuri C6 flow cytometer system and analyzed. Data was presented as relative-fluorescence intensity in a 2-dimensional FACS profile (standardized gating, 20,000 events). Loss of transmembrane potential was shown by shift in peaks in treated cells as compared to control (C– vehicle treated). All experiments were performed in triplicate.