Figure 2

Assessment of type of cell death induced by BT on various ovarian cancer cell lines. (A) Effect of BT on casapses3/7 (columns) and LDH activities (line graph). Caspase 3/7 activity was measured using Caspase-Glo 3/7 Assay kit from Promega and LDH release was measured by using Cyto-Tox-One Homogenous Membrane Integrity kit (Promega). Cells were treated with BT for 6, 24 and 48 hrs. Vehicle treated (media with 1% DMSO) were considered as control against which treated cells were compared. Experiments were performed in triplicate. Data was expressed as mean ± SD of triplicate experiments. *p < 0.05 and **p < 0.01, as compared to control, Students” test. (B) Activation of Caspases 3 and 7 and degradation of PARP as shown by western blot analysis. Ovarian cancer cell lines were treated with BT at 50 μM or 100 μM for 24 hrs. Analysis of the expression of proteins in the lysates of treated and untreated cells (control) was carried out by PAGE and western blot analysis as described (Materials and Methods). Primary antibodies against activated caspase-3, caspase-7 and cleaved PARP were used. As an internal standard for equal loading, blots were probed with an anti -β- actin antibody.