PARP inhibition and the radiosensitizing effects of the PARP inhibitor ABT-888 in in vitrohepatocellular carcinoma models

Table 1 Primer pairs used for gene expression studies and sequencing for single nucleotide polymorphisms genotype studies

Primer name	Primer sequence (5′-3′)	Tm (°C)	NCBI reference sequence
PARP-1 forward	GCCGAGATCATCAGGAAGTATG	62	[GenBank:NM_001618.3]
PARP-1 reverse	ATTCGCCTTCACGCTCTATC	62
PARP-2 forward	GTGGAGAAGGATGGTGAGAAAG	62	[GenBank:NM_001042618.1]
PARP-2 reverse	CTCAAGATTCCCACCCAGTTAC	62
PARP-3 forward	GAGACTACCAGCTTCTCAAGTG	62	[Genbank:NM_001003931.2]
PARP-3 reverse	GTTGCTGCCAGTCTGTTCTA	62
PARG forward	GTCACCGATGGATGTGGATAA	62	[GenBank:NM_003631.2]
PARG reverse	GGGAGGAACTACCATCTTCTTG	62
GAPDH forward	CAAGAGCACAAGAGGAAGAGAG	62	[GenBank:NM_001256799.1]
GAPDH reverse	CTACATGGCAACTGTGAGGAG	62
rs8679 forward	TGTGGGAAGACCAAAGGAAG	52
rs8679 reverse	ATAGAGAAGGCATCTGCATTTTTAAT	52
rs1136410 forward	TTTGCCATTCACTGTGTTGG	55
rs1136410 reverse	TTAATGTCAGTTTTGAGCTTCTCATAG	55

ISSN: 1471-2407