Figure 3
Effect of the ABT-888 treatment and/or radiation treatment in liver cancer cell lines. (A) Inhibition of PARP activity was measured by western blot analysis of pADPr levels after HepG2 cells were treated with 5 μg/ml doxorubicin and/or 10 μM ABT-888 for 2 h. Control treatment was the solvent of ABT-888. (B) Effect of ABT-888 on clonogenic survival of liver cancer cell lines. Cells were treated with 10 μM ABT-888 for 2 h and allowed to grow for 14 days after treatment. (C) Radiation survival of HepG2 (two independent experiments, each in triplicate) and PLC-PRF-5 cells (one experiment in triplicate) without (open symbols) or with (full symbols) ABT-888. The curves were drawn for best fit to the linear-quadratic equation (see Methods). To determine whether the drug-induced modification of radiation response was significant or not, a non-parametric Mann–Whitney U-test was performed on paired sets of data. **, p < 0.001; ***, p < 0.0001 using StatEL (AD Science, Paris, France).