Figure 2

PARP-1 protein expression and PARP activity in liver cells. (A) PARP-1 protein expression was analyzed by western blotting. (B) Densitometry analyses of PARP-1 expression were performed on three independent western blotting experiments. (C) Pearson correlation analysis was performed on the mean of gene and protein expression values and showed a significant positive correlation of PARP-1 protein vs. PARP-1 mRNA expression (Pearson correlation coefficient r = 0.6840, one-tailed p value = 0.0252). (D) PARP activity was measured by western blot analysis of pADPr after cells were treated with 5 μg/ml doxorubicin. HepG2 cells non-treated with doxorubicin were used as a control.