Figure 3

Nf1 ^−/− hematopoietic progenitors of the granulocyte and macrophage lineages are resistant to HQ-induced toxicity. CFU-GM colony counts, relative to untreated controls, are plotted vs. HQ exposure level for cultures derived from both WT and Nf1 ^−/− BM. Bone marrow cells from each genotype were treated with HQ in MethoCult® capable of inducing growth of CFU-GM colonies and then cultured for 12 days. In both genotypes colony formation was reduced upon exposure to as little as 10 μM HQ. CFU-GM numbers continued to fall with increasing HQ exposure up to 30 μM HQ treatment, and at all doses the Nf1 ^−/− BM appeared resistant to HQ-induced toxicity when compared with BM from WT littermates.