Skip to main content
Figure 7 | BMC Cancer

Figure 7

From: Fluoxetine modulates breast cancer metastasis to the brain in a murine model

Figure 7

Fluoxetine upregulates levels of pro-inflammatory cytokines. A) To detect mRNA levels, brain tissue was collected after 3 weeks of fluoxetine treatment. mRNA was isolated, reverse transcribed, and subjected to real-time PCR analysis in order to determine expression levels of several pro-inflammatory markers. Experimental data were normalized to the expression of G3PDH, a housekeeping gene. n = 5 per group, p < 0.05. B) For protein analysis, after 3 weeks of fluoxetine administration, a custom-made mouse cytokine/chemokine panel was used to determine protein concentration of several pro-inflammatory markers in brain extracts from control and treated animals. Analyte expression was normalized to protein concentration in individual samples. n = 6 per group, p < 0.05.

Back to article page