The radiosensitising effect of gemcitabine and its main metabolite dFdU under low oxygen conditions is in vitro not dependent on functional HIF-1 protein

Table 1 mRNA expression profiles of hypoxia-related genes in MDA-MB-231 wt, EV and dnHIF cells

Symbol	Description	Fold up- or downregulation under hypoxia versus normoxia
Symbol	Description	MDA-MB-231 wt	MDA-MB-231 EV	MDA-MB-231 dnHIF
ADM	Adrenomedullin	2.18	1.73	-1.00
ANGPTL4	Angiopoetin-like 4	2.72	3.19	2.65
BHLHE40	Basic helix-loop-helix family, member e40	1.56	2.18	2.16
CASP1	Caspase-1	-1.44	-2.03	-1.67
HIF-1α	Hypoxia inducible factor 1, alpha subunit	-2.61	-3.26	-2.29
HIF-3α	Hypoxia inducible factor 3, alpha subunit	-2.25	-1.71	-1.68
IPCEF1	Interaction protein for cytohesin exchange factors 1	-3.75	-1.51	4.48
LCT	Lactase	-1.02	-2.39	-1.73
LEP	Leptin	-1.96	-1.04	-4.06
MT3	Metallothionein 3	1.52	1.06	2.01
SLC2A4	Solute carrier family 2 (facilitated glucose transporter), member 4	-2.10	-1.16	-1.38

Only genes with a more than two-fold up- or downregulation under hypoxia versus normoxia are presented. (-) represents downregulation. Cells were exposed to normoxia/hypoxia for 24 h (see Methods for full details). All results are from at least 3 independent experiments.

ISSN: 1471-2407