Figure 2

Rab11 and E-cadherin expressions in cultured colon cells. (a) HT-29 and SW480 colon cells were grown on coverslips, cells were fixed and stained with E-cadherin antibody, and E-cadherin was then revealed by Cy2-conjugated secondary antibody and observed by confocal microscopy. (b) HT-29 and SW480 cell lysates were collected and subjected to SDS-page. E-cadherin and Rab11 were detected by Western blot, and GAPDH was used as the internal loading control.