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Figure 1 | BMC Cancer

Figure 1

From: Rab11 regulates E-cadherin expression and induces cell transformation in colorectal carcinoma

Figure 1

E-cadherin and Rab11 expressions in colon tumor tissues. (a) Colon tumor tissue and non-tumor (indicated to be normal) tissue samples from the same patient were processed for immunohistochemistry with antibodies against E-cadherin or Rab11; micrographs were taken using a Zeiss light microscope. Scale bar = 50 μm. (b) E-cadherin and Rab11 expressions in tissues from 9 patients were quantified by “I” index scoring as described in Methods. (c) Protein extracts from colon tumor tissue and non-tumor tissue were subjected to SDS-PAGE and Western blot analysis to determine the expression levels of E-cadherin and Rab11. GAPDH was used as the internal loading control. (d) Quantification of E-cadherin and Rab11 protein expressions in 9 patients by Western blot assay. Value = mean ± SD, * p < 0.05. (e) Immunohistochemistry study of colon tumor tissue showed the E-cadherin and Rab11 expressions in colon tumor infiltrated cells. The asterisk indicates the tumor region, arrows indicate the tumor infiltrated cell nest, and the arrowhead indicates a disarranged gland-like structure. Scale bar = 50 μm. (f) Colorectal tumor and non-tumor (indicated to be normal) tissue paraffin sections from 113 patients were mounted on glass slides as a tissue microarray. E-cadherin and Rab11 were detected by immunohistochemistry. The expressions of E-cadherin and Rab11 were scored and quantified by the “I” index as described in Methods. Value = mean ± SD, * p < 0.05.

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