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Figure 4 | BMC Cancer

Figure 4

From: Combination of hepatocyte specific delivery and transformation dependent expression of shRNA inducing transcriptional gene silencing of c-Myc promoter in hepatocellular carcinoma cells

Figure 4

Estimation of cell survival by trypan blue staining. (A) Trypan blue cell counting assay was done, on the 6thday, post transfection of all AFP promoter/enhancer driven c-Myc shRNA constructs in HepG2 cells. The decrease in cell survival corroborated with the MTT assay and was found to be significant, when compared to their respective scrambled controls (p < 0.05). (B) In the case of Chang Liver cells, significant decrease in the cell viability was observed only by CMVPr – myc (p < 0.001) as the AFP promoter enhancer system was inactive in it.

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