Figure 4From: Combination of hepatocyte specific delivery and transformation dependent expression of shRNA inducing transcriptional gene silencing of c-Myc promoter in hepatocellular carcinoma cells Estimation of cell survival by trypan blue staining. (A) Trypan blue cell counting assay was done, on the 6thday, post transfection of all AFP promoter/enhancer driven c-Myc shRNA constructs in HepG2 cells. The decrease in cell survival corroborated with the MTT assay and was found to be significant, when compared to their respective scrambled controls (p < 0.05). (B) In the case of Chang Liver cells, significant decrease in the cell viability was observed only by CMVPr – myc (p < 0.001) as the AFP promoter enhancer system was inactive in it.Back to article page