Figure 5
Collagen gel contraction assay. Time-course of gel contraction in the presence of HFL-1 transduced with NTC miRNA (solid line), or miRNAs against TGF-β1 (dashed line: TGF-β1 KD), TGF-β2 (dotted line: TGF-β2 KD), or TGF-β1 and TGF-β2 (dashed-dotted line: TGF-β1 + β2 KD). The area of each gel was assessed daily for 5 days and the relative value compared to the initial size was determined. Data shown are the means ± SD of triplicate analyses. Statistical significance was determined by Student’s t-test. *P < 0.05.