Figure 1

Representative western blot analysis of AKT, activated AKTSer473, and PTEN in 38 FL samples (#1-38) and lymphadenitis cases (#39 and #40). AKT was detected in all examined samples, and is shown as a loading control for pAKT detection (panel A). GAPDH is also used as a loading control for PTEN detection (panel B). Asterisks indicate positive samples for pAKT; pAKT expression was detected at high level in samples #6, #7, #9, #13, #14, #29, #31, #32, and #38; and at low level in samples #4, #8, #12, #20, #30, #39, and #40. Jurkat cells served as a positive control for pAKT expression. SupT1 cells served as a positive control for PTEN expression. These results shown are representative of 3 independent experiments.