Figure 6

Up-regulation of GD3 synthase and NEU3 in melanoma cells. (A) Real Time PCR analysis of the mRNA expression of GD3 synthase and (B) of NEU3 sialidase in melanoma cell lines and in melanocytes. Dotted line represents GD3 synthase and NEU3 expression level in adult melanocytes. Values are the mean ± S.D. of four independent experiments. (C) Average mRNA expression of Sp1, assessed by Real Time PCR, in melanoma cell lines and in melanocytes. Values are the mean ± S.D. of four independent experiments. (D) HPTLC separation and (E) percent quantification of melanoma cell (L6) gangliosides before and after incubation with melanoma cell membranes.