Figure 5
From: Molecular subtyping of metastatic melanoma based on cell ganglioside metabolism profiles
CMAH in melanoma cells. (A) RT-PCR analysis of CMAH expression in L6 (cluster 1), L34 (cluster 2), L4 (cluster 3). (B) RT-PCR analysis of deletion at 5′ of CMAH gene in L6 (cluster 1), L34 (cluster 2), L4 (cluster 3). Similar results were obtained for other melanoma cell lines according to the clustering. (C) HPTLC separation of gangliosides of L6 cells after pre-incubation in medium containing 10% FBS or in serum-reduced medium OptiMEM. Three replicate experiments were performed.