Figure 2
From: ΔNp63α enhances the oncogenic phenotype of osteosarcoma cells by inducing the expression of GLI2
Overexpression of ΔNp63α enhanced the malignant phenotype of SaOS-2 cells. A. Analysis of the motilities of SaOS-2- ΔNp63α, SaOS-2-EV and SaOS-2-TAp63α cells in a wound healing assay. Representative photomicrographs of scratch-wounds at the indicated time points after wounding. Arrows indicate the wound width. B. Quantitative analysis of wound closure in cultures of SaOS-2-EV, SaOS-2-ΔNp63α and SaOS-2-TAp63α cells. C. Analysis of anchorage-independent growth in soft agar of SaOS-2-ΔNp63α, SaOS-2-TAp63α and SaOS-2-EV cells. Representative images of colonies stained with 0.005% crystal violet. D. Quantitative analysis of colony formation in soft agar. The numbers of colonies formed by SaOS-2-EV cells were defined as 1. E. Analysis of proliferation of SaOS-2-ΔNp63α cells, SaOS-2-EV and SaOS-2-TAp63α cells using a WST assay. Data shown in 2 B, 2 D and 2E represent the mean ± SEM of three independent experiments ( *P < 0.05).