Figure 1
From: ΔNp63α enhances the oncogenic phenotype of osteosarcoma cells by inducing the expression of GLI2
Analysis of the expression of p63 isoforms in human OS cell lines. The human parental low metastatic (non-invasive) SaOS-2, MG63, HOS and HUO9 cell lines and the respective metastatic (invasive) LM5, M8, 134B and M132 sublines were analysed. A. mRNA levels of p63 splice variants in the indicated OS cancer cell lines were determined using semi quantitative PCR. BxPC3 cells served as the positive control and GAPDH mRNA levels were used as loading controls. M, molecular size marker lane. B. Protein levels of ΔNp63α in the indicated OS cell lines were detected using an antibody specific for ΔNp63.