mRNA and protein expression. (A) Huh7 cells were treated with BBP (1 μM), and mRNA expression of AhR was analyzed by RT-PCR at the indicated time points. The agarose gel image is the expression of AhR mRNA. Each values in the graph was obtained by densitometry and is the mean of three independent experiments. Each value in the graph is the mean ± SD from three independent experiments. The asterisks indicate a significant difference expression relative to the level at 0 minute, as analyzed by Student’s t-test (p < 0.05). (B) Huh7 cells were treated with BBP (1 μM) or DMSO as the control group for 15 minutes, and then AhR mRNA was stained by fluorescence (carboxyfluorescein, FAM) in situ hybridization. Imaging was performed by confocal microscopy. (C) Huh7 cells were treated with BBP (1 μM), and then AhR levels were analyzed by immunoblotting at the indicated time.