E-cadherin loss compromises cell migration of MCF10A
CDH1-/-. Both isogenic cell lines were grown to confluence in complete media, wounds were generated using the 96-well WoundMaker and the data collected over 35 h and analyzed on the IncuCyte. a) Representative wounds on MCF10A isogenic cells under different ECM conditions at the start (immediately after wounding) and 14 h post wounding. b) The time course of cell migration was quantified using wound confluence at 1 h intervals over 35 h. MCF10A CDH1-/- cells were shown to take significantly longer in wound closing compared to wildtype cells under different coating conditions.