E-cadherin loss altered cytoskeletal organization in MCF10A
cells. a) Loss of E-cadherin altered tubulin cytoskeletal arrangement. On the apical surface of MCF10A cells, the microtubules showed radial pattern of organization (indicated by white arrows) with the minus end densely anchored in the centre and the plus end extending towards the cell cortex. However, in MCF10A CDH1 -/- cells had gross defect in the radial pattern of organization and often oriented parallel to the cortex (indicated by white arrows). At the basal surface, the microtubules form a meshwork like structure with no striking difference observed between the two cell lines. b) Loss of E-cadherin altered actin cytoskeletal arrangement. On the apical surface of MCF10A cells, actin forms cross-linking filamentous meshwork while basally it organizes itself into stress fibres like structure. Overall apical actin meshwork looks similar in both MCF10A isogenic cells but basally there are more and thicker stress fibres in MCF10A CDH1-/- cells (indicated by white arrows).