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Figure 4 | BMC Cancer

Figure 4

From: Pharmacological modulation of autophagy enhances Newcastle disease virus-mediated oncolysis in drug-resistant lung cancer cells

Figure 4

Knockdown of ATG5 enhances NDV/FMW-mediated oncolysis in A549/DDP cells. A549/DDP and A549/PTX as well as parental cells were transfected with either specific siRNA targeting ATG5 or scrambled siRNA. (A) At 72 h after transfection, the levels of ATG5 and β-Actin expression were measured by immunoblot analysis. (B) At 48 h after transfection, cells were infected with NDV/FMW (MOI =10) for 24 and 48 h respectively. Cell death was determined by MTT assay. Bar graph summarized the percentage of cell death from three independent experiments (*p < 0.05, **p < 0.01). (C, D) A549/DDP cells were transfected with either ATG5 siRNA or scrambled siRNA for 48 h, cells were then infected with NDV/FMW (MOI =10) or mock-infected for indicated times. (C) Cell lysates at 24 hpi was analysed by immunoblot for ATG5, cleaved caspase-3 and β-Actin, while cell-free supernatant was collected and determined by immunoblot for high-mobility group box1(HMGB1) (D). At 24 and 48 hpi, cells were double-stained with annexin V-FITC and propidium iodide (PI), apoptosis was assessed by FACS analysis. Data shown are representative of three independent experiments.

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