Figure 2

Autophagic signaling pathways are regulated in response to NDV/FMW infection in drug-resistant lung cancer cells. A549/DDP and A549/PTX as well as parental cells were infected with NDV/FMW at an MOI of 10 at the indicated time points. Expression of beclin-1 (B), total and phosphorylated (p-) Akt, mTOR and p70S6K (A) was analyzed by immunoblot, using β-Actin as a loading control. R stands for rapamycin, an autophagy inducer inhibiting mTOR phosphorylation. The ratios of phosphorylated protein to β-Actin are presented below the blots. Results shown are representative of three independent experiments.