Figure 5

In vivo anti-proliferation property of AB215 . Athymic nude mice implanted with continuous releasing E2 pellet were xenografted with MCF7 cells in the dorsal region. Low (0.12 mg/kg · injection) or high (0.4 mg/kg · injection) dose of ligand or 0.6 mg/kg tamoxifen was injected in q2d × 10 (every 2 days for 10 weeks) schedule. A) Collected tumors were measured for volume according to π/6 × (length) × (width) × (height) equation (n = 5). Results are presented in means + SD. B) Collected tumors were analyzed to determine levels of nuclear proliferation marker Ki67 (final magnification 400×). Each section was counter stained with hematoxylin (blue/purple) to distinguish proliferating and non-proliferating cells. C) The quantification of the images from two different tumor sections representing the total number of cells and the number of cells labeled with Ki67.