Figure 4

AB215 inhibits estrogen induced signaling. To investigate the effect of AB215 on Estrogen signaling, A) mRNA levels of TFF1, c-myc, Bcl2 and VEGF and B) protein levels of TFF1, c-myc, Bcl2 were analyzed in MCF7 cells. Cell were exposed to the treatment (500 ng/ml AB215 and 10nM E2) for 48 hours (TFF1, Bcl2, and VEGF) and 2 hours (c-myc). qRT-PCR and western blot analysis was performed as described in Figure 3 and experimental conditions are same as in Figure 2i. mRNA levels are presented as means + SD of quadruplicate, n = 3 and their significance has been analyzed by one-way ANOVA (* = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001).