Figure 2

Loss of BRAT1 induces morphological changes and in vivo tumor growth. (A) The injury lines were made on confluent monolayers of control (NC) and BRAT1 knockdown (sh3 and sh8) HeLa cells. Wound healing potential was analyzed under a light microscope (×40) at indicated time points. (B) Both control (NC) and BRAT1 knockdown (sh2 and sh20) MDA-MA-231 cells were seeded onto migration chamber and infiltrated cells were stained with 0.1% crystal violet, counted and then quantified (bottom). Percentage of migration was expressed as ratio of migrating cells numbers of knockdown cells to control cells. The expression of BRAT1 was confirmed by immunoblot (inserts) Actin protein was used as internal control. (C) Control and BRAT1 knockdown HeLa cells were injected into female nude mice as described in M&M. Tumor size was measured at indicated days and then sacrificed at day 27. Tumors were isolated from individual mouse and photographs were taken. Data were representative of three independent experiments.