API5 and pERK1/2 expression in human cervical neoplasias specimens. (A) Representative immunohistochemical staining images of API5 and pERK1/2 in normal, low grade CIN, high grade CIN, and cervical cancer tissues. Bars: 100 μm. (B) API5 IHC staining score in cervical neoplasia samples. API5 IHC staining score in cervical cancer samples was significantly higher than that of all other groups. The mean API5 score associated directly with each tumor stage, stage I tumors stained more weakly than stage II and stage IV tumors. API5 IHC staining score in poorly differentiated cervical cancer samples was significantly higher than that of well/moderately differentiated cancers.