Figure 5From: Expression of miR-34c induces G2/M cell cycle arrest in breast cancer cells Evaluation of miR-34c targets. Following transfection of MDA-MB-231, MDA-MB-468 and BT-549 (breast cancer cells with miR-34c mimic or negative control for 96Ā h, cells were analyzed for expression of PRKCA (B), CCND1 (C), CDK4 (D), CDK6 (E) or CDC23 (G) mRNA with real-time quantitative PCR or for protein expression with Western blot (A and F). Data represent meanāĀ±āSEM from 2ā3 independent experiments and the blots shown are representative of three independent experiments. Asterisks indicate statistically significant differences (* pā<ā0.05, ** pā<ā0.01, *** pā<ā0.001, Studentās t-test) compared to control cells.Back to article page