Figure 4

Effect of miR-34c on apoptosis of breast cancer cells. MDA-MB-231 (A), MDA-MB-468 (B) and BT-549 (C) breast cancer cells were transiently transfected with either miR-34c mimic or negative control for 96 h. After 96 h, cells were subjected to Annexin V-APC staining and flow cytometry analysis. Data (mean ± SEM, n = 2-3) represent percent AnnexinV-positive cells with miR-34c related to scramble treatment. Asterisks indicate statistically significant differences (* p < 0.05, Student’s t-test) compared to control cells.