Figure 9

Co-ordinated expression of miRNA biogenesis proteins. A, Dicer and TARBP2 protein expression in SKBR3 cells after transient transfection with siRNA targeting Dicer or control siRNA. Results show three technical replicates per treatment. B, TARBP2 and Dicer protein expression in SKBR3 cells after transient transfection with siRNA targeting TARBP2 or control siRNA. C, Changes in Dicer and TARBP2 protein expression after exposure to 16, 24 and 48 h of hypoxia. D, Dicer and Drosha protein expression in SKBR3 cells after transient transfection with siRNA targeting Dicer or control siRNA. Results show three technical replicates per treatment. E, Dicer and Drosha protein expression in SKBR3 cells after transient transfection with siRNA targeting Drosha or control siRNA. Results show three technical replicates per treatment. F and G, total protein on PVDF membranes. H and I, Densitometric analysis of D and E following normalisation with total protein on PVDF membrane. Protein levels were examined by immunoblotting. α-actinin, β-tubulin or total protein on the PVDF membrane were used as the loading controls.