Figure 7
From: Hypoxia represses microRNA biogenesis proteins in breast cancer cells
Dicer repression by miR-103 and miR-107 in hypoxia. Expression of miR-103 and miR-107 were examined in MCF7 cells, after exposure to 0.1% O2 for different durations. A, miR-103 expression in MCF7 cells after hypoxia (0.1% O2 for 16, 24 and 48 h) vs. normoxia (P = 0.03). B, miR-107 expression in MCF7 cells after hypoxia (0.1% O2 for 16, 24 and 48 h) vs. normoxia (P = 0.04). * denotes p < 0.05 compared with parallel controls. Data represent normalized mean ± S.E (error bars) (n = 3). miRNA levels were analysed by q-RT PCR and normalised to U6 levels. Statistical significance established by Student’s t-test. Dicer expression was examined after transient transfection with miR-103/107 inhibitors and exposure to hypoxia vs. normoxia. C, Dicer protein expression after transfection of MCF7 cells with miR-103/107 inhibitors or control inhibitors and exposure to hypoxia (0.1% O2 for 48 h) vs. normoxia. Results show three technical replicates per treatment. Dicer and α-actinin protein levels were examined by immunoblotting. α-actinin was used as the loading control.