Figure 6

Dicer expression in not regulated by miR-210 in hypoxia. Dicer expression was examined after transient transfection with a miR-210 mimic and miR-210 antagomir in SKBR3 cells. A, miR-210 expression in SKBR3 cells after transient transfection with control mimic and miR-210 mimic and miR-210 antagomir in normoxia. B, Dicer mRNA expression in SKBR3 cells after transient transfection with control mimic and miR-210 mimic. C, Dicer protein expression in SKBR3 cells after transfection with control mimic and miR-210 mimic. Results show three technical replicates per treatment. D, Dicer mRNA expression in SKBR3 cells after transient transfection with control mimic, miR-210 mimic and miR-210 inhibitor, then exposed to 1% O₂ for 72 h. Data represent normalized mean ± S.E (error bars) (n = 3). mRNA levels were analysed by RT-PCR and normalised to β-2-microglobulin mRNA levels. Statistical significance established by Student’s t-test. Dicer and β-tubulin protein levels were examined by immunoblotting. β-tubulin was used as the loading control.