Figure 4
From: Hypoxia represses microRNA biogenesis proteins in breast cancer cells
Dicer repression in hypoxia is not HIF dependent. Dicer expression was examined after HIF-1α and HIF-2α inhibition with siRNA in hypoxia compared to normoxia A, HIF-1α protein expression in SKBR3 cells after transient transfection with a HIF-1α targeting siRNA or control siRNA, then exposure to hypoxia (0.1% O2 for 24 h) vs. normoxia. Results show two technical replicates per treatment. B, Dicer mRNA expression in MCF7 cells after transient transfection with HIF-1α, HIF-2α or control siRNA, then exposure to hypoxia (0.1% O2 for 48 h) vs. normoxia. Data represent normalized mean ± S.E (error bars) (n = 3). Dicer mRNA levels were analysed by RT q-PCR and normalised to β-2-microglobulin mRNA levels. C, Dicer protein expression in SKBR3 cells after transient transfection with HIF-1α targeting siRNA or control siRNA, then exposure to hypoxia (0.1% O2 for 48 h) vs normoxia. Results show two technical replicates per treatment. Dicer and α-actinin protein levels were examined by immunoblotting. α-actinin was used as the loading control.