Figure 2
From: Hypoxia represses microRNA biogenesis proteins in breast cancer cells
Dicer mRNA expression in hypoxia vs. normoxia. Dicer mRNA expression was examined in MCF7 and HT29 cells after exposure to 0.1% O2 for different durations. A, Dicer mRNA expression in MCF7 cells after exposure to 0.1% O2 for 8 h (P = 0.0007), 16 h (P = 0.0009), 24 h (P = 0.02) and 48 h (P = 0.008). B, Dicer mRNA expression in HT29 cells after exposure to 0.1% O2 for 16 h (P = 0.01), 24 h (P = 0.02) and 48 h (P = 0.09). *denotes P < 0.05 compared with parallel controls in normoxia. Data represent normalized mean ± S.E (error bars) (n = 3). Dicer mRNA levels were analysed by RT-PCR and normalised to 18S rRNA levels. Statistical significance established by Student’s t-test.